An immunochemical approach for the determination of trace amounts of enantiomeric impurities

Authors
Hofstetter, O Hofstetter, H Wilchek, M Schurig, V Green, BS
Citation
O. Hofstetter et al., An immunochemical approach for the determination of trace amounts of enantiomeric impurities, CHEM COMMUN, (17), 2000, pp. 1581-1582
Citations number
21
Categorie Soggetti
Chemistry
Journal title
CHEMICAL COMMUNICATIONS
ISSN journal
13597345 → ACNP
Issue
17
Year of publication
2000
Pages
1581 - 1582
Database
ISI
SICI code
1359-7345(2000):17<1581:AIAFTD>2.0.ZU;2-W
Abstract
The application of stereoselective antibodies in an enzyme immunoassay enab les the quantitative determination of enantiomeric impurities beyond the ou ter limits of currently available methods; thus, using an antibody raised a gainst a derivative of d-phenylalanine, the d-enantiomer of the free amino acid can be detected in a 100000 fold excess of the L-enantiomer (ee 99.998 %).