MAP-H2O2-HRP voltammetric enzyme-linked immunoassay for trace southern bean mosaic virus

A voltammetric enzyme-linked immunoassay based on the new system of nz-amin ophenol (MAP)-H2O2-horseradish peroxidase(HRP) has been developed and used for the detection of HRP and southern bean mosaic virus(SBMV). HRP or label led HRP catalyzes the oxidation reaction of MAP with H2O2, the product of w hich produces a sensitive voltammetric peak at potential of -0.46 V(vs. SCE ) in Britton-Robinson (B-R) buffer solution, By using this voltammetric pea k, HRP can be measured with a detection limit of 3.8 x 10(-9) g/L and a lin ear range of 1.0 x 10(-8)-1.0 x 10(-6) g/L. The detection limit for the cla rified SBMV is 4.0 ng/mL and the linear range of 4.0-5 000 ng/mL. The highe st dilution ratio detected for the infected leaf sap is 1 : 1.5 x 10(5).