Evidence for Ca2+ activation and inactivation sites on the luminal side ofthe cardiac ryanodine receptor complex

We have used tryptic digestion to determine whether Ca2+ can regulate cardi ac ryanodine receptor (RyR) channel gating from within the lumen of the sar coplasmic reticulum (SR) or whether Ca2+ must first flow through the channe l and act via cytosolically located binding sites. Cardiac RyRs were incorp orated into bilayers, and trypsin was applied to the luminal side of the bi layer. We found that before exposure to luminal trypsin, the open probabili ty of RyR was increased by raising the luminal [Ca2+] from 10 mu mol/L to 1 mmol/L, whereas after luminal trypsin exposure, increasing the luminal [Ca 2+] reduced the open probability. The modification in the response of RyRs to luminal Ca2+ was not observed with heat-inactivated trypsin, indicating that digestion of luminal sites on the RyR channel complex was responsible, Our results provide strong evidence for the presence of luminally located Ca2+ activation and inhibition sites and indicate that trypsin digestion le ads to selective damage to luminal Ca2+ activation sites without affecting luminal Ca2+ inactivation sites. We suggest that changes in luminal [Ca2+] will be able to regulate RyR channel gating from within the SR lumen, there fore providing a second Ca2+-regulatory effect on RyR channel gating in add ition to that of cytosolic Ca2+. This luminal Ca2+-regulatory mechanism is likely to be an important contributing factor in the potentiation of SR Ca2 + release that is observed in cardiac cells in response to increases in int ra-SR [Ca2+].