Refined deletion mapping in sporadic breast cancer at chromosomal region 8p12-p21 and association with clinicopathological parameters

We have further refined the loss of heterozygosity (LOH) pattern on the hum an chromosomal region 8p12-p21 using 15 well characterised microsatellite m arkers in a panel of 50 breast carcinomas. The allelic loss pattern of thes e rumours suggests the presence of five commonly deleted regions on 8p12-p2 1. The most commonly deleted region was located between markers D8S1734 and D81989, spanning a distance of approximately 3 cM and reaching 56% LOH at locus NEFL. LOH at 8p12-p21 was significantly correlated with large tumour size (T > 5 cm). Patients with the age at diagnosis of breast cancer betwee n 45 and 55 years showed significantly more LOH than patients older than 55 years or younger than 45 years. No correlation was observed between 8p12-p 21 alterations and histological tumour type, grade and the presence of lymp h node metastases. (C) 2000 Elsevier Science Ltd. All rights reserved.