The aim of this study was to evaluate, using high-performance liquid chroma
tography, the concentration of ceftazidime in agar released from an E test
strip, sampling at the edge of the strip at different points (1, 2, 4, 8, 1
6, 32, 64, and 128 mu g/ ml) at 6, 15, and 24 h after its deposition on uni
noculated plates. From 6 to 24 h, the ceftazidime concentration in agar inc
reased at the graduations 1, 2, and 4 mu g/ml (+140, +82, and +58%, respect
ively), remained fairly constant at 8 mu g/ml (-1.9%), and decreased at 16,
32, 64, and 128 mu g/ml (-25, -44, -36, and -58%, respectively). In the 6-
24 h range, the ceftazidime concentrations between 16 and 1 mu g/ml were +/
-1 serial dilution of the values reported on the strip, confirming the accu
racy of the E test in agar.