Needle-type glucose microbiosensor based on glucose oxidase immobilised inan overoxidised polypyrrole film (an in-vitro study)

A fast response, needle-type glucose micro-biosensor has been fabricated by a one-step electrochemical immobilisation of glucose oxidase in a polypyrr ole film. The sensor shows a remarkable rejection of electroactive interfer ences, especially paracetamol. The maximum bias observed in the worst situa tion never exceeded the value of 6%. The fabrication procedure delivered ve ry reproducible devices and the sensitivity of a newly prepared biosensor w as typically 650 nAmM(-1)cm(-2). The kinetic parameters, obtained from an e xisting model, permitted to understand the sensor behaviour.