Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?

In reptiles as in other vertebrates, multiple forms of gonadotropin-releasi ng hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by di rect or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH- II), salmon GnRH (sGnRH) and several unidentified GnRH-like forms. In the p resent study GnRH variants were investigated in brain extracts of the lizar d Tupinambis teguixin (= T. merinae) by combining high-performance liquid c hromatography (RP-HPLC) followed by radioimmunoassays (RIA). Two peaks show ing GnRH immunoreactivity with the elution position of synthetic mammalian GnRH (mGnRH) and cGnRH-II were detected. Both peaks were further analyzed w ith different radioimmunoassay systems specific for mGnRH, cGnRH-I, and cGn RH-II. Pooled fractions corresponding to the first eluting peak showed no c rossreactivity when analyzed with a cGnRH-I specific assay and legit-log di splacement curves were not significantly different from those of synthetic mGnRH with homologous RIA systems. The second peak showed immunological cha racteristics of cGnRH-II when analyzed with a specific antiserum. The first ir-GnRH peak was selected for further RP-HPLC purification showing similar chromatographic behavior as mGnRH synthetic standard. We demonstrated the absence of cGnRH-I in this lizard using well-characterized antisera. (C) 20 00 Academic Press.