W. Morelle et al., Phosphorylcholine-containing N-glycans of Trichinella spiralis: identification of multiantennary lacdiNAc structures, GLYCOBIOLOG, 10(9), 2000, pp. 941-950
Although the presence of phosphorylcholine (PC) in Trichinella spiralis is
well established, the precise structure of the PC-bearing molecules is not
known. In this paper, we report structural studies of N-glycans released fr
om T.spiralis affinity-purified antigens by peptide N-glycosidase F. Three
classes of N-glycan structures were observed: high mannose type structures;
those which had been fully trimmed to the trimannosyl core and were sub-st
oichiometrically fucosylated; and those with a trimannosyl core, with and w
ithout core fucosylation, carrying between one and eight N-acetylhexosamine
residues. Of the three classes of glycans, only the last was found to be s
ubstituted with detectable levels of phosphorylcholine.