K. Udaka et al., An automated prediction of MHC class I-binding peptides based on positional scanning with peptide libraries, IMMUNOGENET, 51(10), 2000, pp. 816-828
Specificities of three mouse major histocompatibility complex (MHC) class I
molecules, K-b, D-b, and L-d, were analyzed by positional scanning using c
ombinatorial peptide libraries. The result of the analysis was used to crea
te a scoring program to predict MHC-binding peptides in proteins. The capac
ity of the scoring was then challenged with a number of peptides by compari
ng the prediction with the experimental binding. The score and the experime
ntal binding exhibited a linear correlation but with substantial deviations
of data points. Statistically, for approximately 80% of randomly chosen pe
ptides, MHC-binding capacity could be predicted within one log concentratio
n of peptides for a half-maximal binding. Known cytotoxic T-lymphocyte epit
ope peptides could be predicted, with a few exceptions. Tn addition, freque
nt findings of MHC-binding peptides with incomplete or no anchor amino acid
(s) suggested a substantial bias introduced by natural antigen processing i
n peptide selection by MHC class I molecules.