Random transposon mutagenesis of Campylobacter jejuni

Genetic studies of Campylobacter jejuni have been limited due to the lack o f a transposon mutagenesis method. Here, we describe a novel technique for random transposon mutagenesis using a mariner-based transposon into C. jeju ni strain 480. Insertions were random, as demonstrated by Southern blot ana lysis and insertional junction sequencing. We have demonstrated, for the fi rst time, random in vivo transposon mutagenesis of C. jejuni.