Influences of everninomicin, vancomycin and teicoplanin on chemical mediator release from rat peritoneal mast cells

We examined influences of certain antibiotics on the release of chemical me diators from isolated rat peritoneal mast cells in vitro. Isolated peritone al mast cells were obtained from male Wistar strain rats. Everninomicin (0. 06 - 1.2 mg/ml), vancomycin (0.05 - 1.0 mg/ml), teicoplanin (0.07 - 1.4 mg/ ml) and concanavalin A (0.01 mg/ml) were used. Isolated mast cells were inc ubated in the presence of various concentrations of the test compound at 37 degrees C for 10 min. Histamine contents of the supernatant and cell pelle t were measured by an automated fluorometric method. Prostaglandin D-2 (PGD (2)) contents of the supernatant were determined by enzyme immunoassay. Eve rninomicin (0.06-1.2 mg/ml) had no influence on histamine and PGD2 release from mast cells. On the other hand, vancomycin significantly released both histamine (0.5 and 1.0 mg/ml) and PGD(2) (1.0 mg/ml) from mast cells, but v ancomycin did not affect concanavalin A-induced histamine and PGD(2) releas e. Teicoplanin (more than 0.07 mg/ml) significantly stimulated histamine an d PGD(2) release from mast cells and it also significantly potentiated conc anavalin A-induced histamine and PGD:! release. These results suggest that everninomicin causes no chemical mediator release from mast cells, differen t from vancomycin and teicoplanin.