Volatile chemicals identified in extracts from leaves of Japanese mugwort (Artemisia princeps Pamp.)

Extracts from leaves of Japanese mugwort (Artemisia princeps Pamp.) were ob tained using two methods: steam distillation under reduced pressure followe d by dichloromethane extraction (DRP) and simultaneous purging and extracti on (SPSE). A total of 192 volatile chemicals were identified in the extract s obtained by both methods using gas chromatography (GC) and gas chromatogr aphy-mass spectrometry (GC-MS). They included 47 monoterpenoids (oxygenated monoterpenes), 26 aromatic compounds, 19 aliphatic esters, 18 aliphatic al cohols, 17 monoterpenes (hydrocarbon monoterpenes), 17 sesquiterpenes (hydr ocarbon sesquiterpenes), 13 sesquiterpenoids (oxygenated sesquiterpenes), 1 2 aliphatic aldehydes, 8 aliphatic hydrocarbons, 7 aliphatic ketones, and 9 miscellaneous compounds. The major volatile constituents of the extract by DRP were borneol (10.27 ppm), alpha-thujone (3.49 ppm), artemisia alcohol (2.17 ppm), verbenone (1.85 ppm), yomogi alcohol (1.50 ppm), and germacren- 4-ol (1.43 ppm). The major volatile constituents of the extract by SPSE wer e 1,8-cineole (8.12 ppm), artemisia acetate (4.22 ppm), alpha-thujone (3.20 ppm), beta-caryophyllene (2.39 ppm), bornyl acetate (2.05 ppm), borneol (1 .80 ppm), and trans-beta-farnesene (1.78 ppm).