Enzymatic method for the determination of inorganic and organic inhibitorsof alcohol dehydrogenase

The inhibiting effect of heavy metal ions, organic nitrogen-containing hete rocyclic compounds, and amino acids on the catalytic activity of yeast alco hol dehydrogenase (ADH) in the reaction of ethanol oxidation by nicotinamid e adenine dinucleotide was detected. Sensitive procedures were developed fo r the determination of the most effective ADH inhibitors [mercury(II), silv er(I), zinc(II), copper(II), 1,10-phenanthroline, 2,2'-dipyridyl, 8-hydroxy quinoline, quinoline, 3,4-dimethylimidazole, benzimidazole, 1,2,3-benzotria zole, histidine, tryptophan, proline, and histamine] with c(L) = 5 x 10(-10 )-1 x 10(-4) M (RSD = 1-12%).