Covalent immobilization of glucose oxidase to Poly(O-Amino benzoic acid) for application to glucose biosensor

A biosensor for glucose utilizing glucose oxidase (GOX) covalently coupled to poly(o-amino benzoic acid) (PAB; a carboxy-group-functionalized polyanil ine) is described. Amperometric response measurements conducted via unmedia ted and mediated (with ferrocene carboxylic acid and tetrathiafulvalene) re oxidation of GOX show that glucose can be detected over a wide range of con centrations. An enzyme-conducting polymer-mediator model provides for bette r charge transport in a biosensor. The optimal response, obtained at pH 5.5 and 300 K, lies in the 1-40 mM range. A kinetic plot yields the value of t he apparent Michaelis-Menten constant, K-m(app). The operational stability of the PAB-based glucose biosensor was experimentally determined to be abou t 6 days. (C) 2000 John Wiley & Sons. Inc.