Mutations in the estrogen receptor ligand binding domain discriminate between hormone-dependent transactivation and transrepression

The estrogen receptor (ER) suppresses transcriptional activity of the Re1A subunit of nuclear factor-KB in a hormone-dependent manner by a mechanism i nvolving both the receptor DNA binding domain and ligand binding domain (LB D). In this study we examine the role of the ER LED in mediating ligand-dep endent RelA transrepression. Both ER alpha and ER beta inhibit RelA in resp onse to 17 beta-estradiol but not in the presence of antihormones. We have identified residues within the ER alpha LED that are responsible for recept or dimerization and show that dimerization is necessary for transactivation and transrepression. Moreover we have generated mutant receptors that have lost their ability to inhibit RelA but retain their capacity to stimulate transcription and conversely mutants that are transcriptionally defective b ut capable of antagonizing Re1A Overexpression of p160 and cAMP-response el ement-binding protein-binding protein/p300 co-activators failed to relieve repression of RelA, which is consistent with the demonstration that RelA in hibition can occur independently of these co-activators. These findings sug gest it is unlikely that sequestration of these cofactors required for ER t ranscriptional activation can account for hormone-dependent antagonism of R e1A The identification of ER mutants that discriminate between transactivat ion and transrepression implies that distinct surfaces within the LED are i nvolved in mediating these two receptor functions.