Photodynamic biologic tissue glue in perforating rabbit corneal wounds

Objective: To evaluate the in vivo wound modulating effects of a new biolog ic tissue adhesive, Background Data: Biological tissue glue can serve as an important adjunct technology in the treatment of a number of ocular condit ions, including closure of corneal wounds. These experiments were performed to evaluate the wound healing effects of a new photodynamic biologic tissu e glue (PBTG) in perforating rabbit corneal incisions, Methods: Perforating corneal wounds were created in 12 pigmented rabbits. Two perforating incis ions were made 180 degrees apart 2 mm from the limbus, One incision in each eye was closed with a single interrupted 10-0 nylon suture, and the other closed with PBTG, Rabbits were followed biomicroscopically and sacrificed a t 1-, 3-, and 7-week intervals for histological evaluation of their corneal wounds, Results: There were no clinical signs of PBTG-related toxicity in any of the rabbits. Compared to control wounds, all glued corneas had great er epithelial filling, less stromal edema, and fewer inflammatory cells at the 1-, 3-, and 7-week stages and showed greater overall wound maturation. Conclusion: Our data suggest that PBTG is an effective and nontoxic tissue adhesive that accelerates normal corneal wound healing.