Tyrosine hydroxylase-expressing and/or aromatic L-amino acid decarboxylase-expressing neurons in the mediobasal hypothalamus of perinatal rats: Differentiation and sexual dimorphism

In this quantitative and semiquantitative immunocytochemical study, the aut hors evaluated the differentiation of neurons expressing tyrosine hydroxyla se (TH) and/or aromatic L-amino acid decarboxylase (AADC) in the mediobasal hypothalamus (MBH) of male and female rats on embryonic day 18 (E18), E20, and postnatal day 9 (P9). Four neuronal populations were distinguished acc ording to either enzyme expression or neuron location. The earliest and mos t prominent first population was represented by TH-immunoreactive (IR)/AADC -immunonegative (IN) neurons that were detected initially at E18 and always were located in the ventrolateral region of the MBH. The second population of TH-IN/ AADC-IR neurons was observed first at E20 and, after that time, was distributed dorsomedially. The third minor population of TH-IR/AADC-IR neurons initially was detected at E20 and was located dorsomedially. The fo urth population was represented by TH-IR/AADC-IN neurons that were distribu ted in the dorsomedial region at any studied age. The numbers of TH-IR and AADC-IR neurons increased from their initial detection at E18 and E20 until P9. The area of TH-IR and AADC-IR neurons also increased from E18 to E20 a nd from E20 to P9, respectively. Both TH-IR and AADC-IR neurons showed sex differences in the neuron number, size, and optic density (OD). The numbers of TH-IR neurons in males exceeded those of females at F20 and at P9, alth ough, at P9, sexual dimorphism was a characteristic only of the ventrolater al population. The area and OD of TH-IR neurons from females exceeded those from males in the entire mediobasal hypothalamus (MBH) at E18 and E20 but only in its dorsomedial region at P9. Sexual dimorphism also was an attribu te of AADC-IR neurons at E20 and P9. Their number, size, and OD were signif icantly higher in females than in males. Thus, the MBH of perinatal rats co ntained two major populations of TH-IR/AADC-IN or TH-IN-AADC-IR neurons and a minor population of TH-ITC/AADC-IR neurons. The differentiating neurons expressing either enzyme showed sexual dimorphism. J. Comp. Neurol. 425: 16 7-176, 2000. (C) 2000 Wiley-Liss, Inc.