Six pyrethrin esters were separated from whole pyrethrum by using high perf
ormance liquid chromatography on a silica column. Dilutions of individual e
sters were applied topically to house flies (Musca domestica L.) and compar
ed to whole pyrethrum and transpermethrin. LD50's averaged from two signifi
cant dosage-mortality regressions per chemical were in ascending toxicity:
Cinerin 1 (1.77 mu g/fly), jasmolin 1 (1.28 mu g/fly), pyrethrin Il (0.49 m
u g/fly), jasmolin 11 (0.46 mu g/fly), cinerin 11 (0.43 mu g/fly), pyrethri
n 1 (0.20 mu g/fly), 25% pyrethrin extract (0.11 mu g/fly) and trans-permet
hrin (0.0072 mu g/fly).