Effect of pyriproxyfen and photoperiod on free amino acid concentrations and proteins in the hemolymph of the Colorado potato beetle, Leptinotarsa decemlineata (Say)

North Dakota strain of the Colorado potato beetle, Leptinotarsa decemlineat a (Say), was reared under both short- (8L:16D) and long-day (17L:7D) condit ions. Age-related and pyriproxyfen- (JHA-) induced changes in hemolymph fre e amino acids and proteins were examined. Under a short-day photoperiod, th e total free amino acid concentration in the hemolymph increased gradually up to 20 days of adult life, but the long-day beetles showed marked increas es during the first 10 days and then decreased afterwards. Proline, glutami ne and valine were the most abundant free amino acids in both sexes of beet les held under either short or long-day photoregims. JHA treatment of diapa using adults, held under either short- or long-day conditions after treatme nt, terminated diapause as indicated by re-emergence from the vermiculite, feeding, mating, changes in foe amino acid levels, the disappearance of dia pause protein 1 and appearance of vitellogenin in the hemolymph. Furthermor e, most of the JHA-treated females held under long-day conditions also matu red oocytes and oviposited, but those held under short-day conditions did n ot. Published by Elsevier Science Ltd.