Detection of expanded T cell clones in skin biopsy samples of patients with lichen sclerosus et atrophicus by T cell receptor-gamma polymerase chain reaction assays
A. Lukowsky et al., Detection of expanded T cell clones in skin biopsy samples of patients with lichen sclerosus et atrophicus by T cell receptor-gamma polymerase chain reaction assays, J INVES DER, 115(2), 2000, pp. 254-259
Lichen sclerosus et atrophicus is a chronic dermatosis of unknown etiology
and pathogenesis. Lichen sclerosus et atrophicus associated skin lesions sh
ow T cell enriched infiltrates, sometimes resembling the histologic picture
of early mycosis fungoides. It is supposed that the infiltrating T cells p
articipate in the pathogenesis of atrophy and sclerosis. We investigated sk
in biopsies from 39 lichen sclerosus et atrophicus patients by histology, i
mmunohistochemistry and, in order to establish the status of T cell clonali
ty, by polymerase chain reaction amplifying the T cell receptor-gamma rearr
angements. A stage-dependent shift of the CD3-positive T cells was observed
from a predominantly CD4-positive to a predominantly CD8-positive phenotyp
e. The increase of CD8-positive cells was associated with more pronounced e
pidermotropism and basal degeneration. Nearly all CD8-positive cells expres
sed cytotoxic granules (TIA1), possibly causing the basal destruction. In t
he late fibrotic stage of the disease, only a weak or no infiltrate was fou
nd. Regarding the T cell receptor-gamma polymerase chain reaction, the pres
ence of clonally expanded T cells was demonstrated in 19 of 39 patients (49
%) by at least one of two different high resolution electrophoresis techniq
ues applied to separate the amplification products. Thus, for the first tim
e clonally expanded infiltrating T cells were detected in lichen sclerosus
et atrophicus. Furthermore, this is one of the first reports on the detecti
on of clonally expanded infiltrating T cells in an inflammatory skin diseas
e. The clonal T cells could not be assigned to the CD4 or CD8 subtype. Most
likely, their presence is not the result of a malignant transformation but
a response to an as yet unknown lichen sclerosus et atrophicus associated
antigen.