Expression of cutaneous lymphocyte-associated antigen regulated by a set of glycosyltransferases in human T cells: Involvement of alpha 1,3-fucosyltransferase VII and beta 1,4-galactosyltransferase I
F. Nakayama et al., Expression of cutaneous lymphocyte-associated antigen regulated by a set of glycosyltransferases in human T cells: Involvement of alpha 1,3-fucosyltransferase VII and beta 1,4-galactosyltransferase I, J INVES DER, 115(2), 2000, pp. 299-306
Cutaneous lymphocyte-associated antigen (CLA), which plays a key part in sk
in homing of human CD4(+) memory T cells via CLA/E-selectin binding, is upr
egulated by IL-12 and downregulated by IL-4. Although alpha 1,3-fucosyltran
sferase VII is essential for synthesis of the CLA carbohydrate epitope, lit
tle is known about how the CLA expression is regulated by a number of glyco
syltransferases. A 6 wk long-term culture for the in vitro differentiation
of naive Th cells to memory Th1 cells was employed. By repeated activation
in the presence of IL-12, naive T cells differentiated into memory Th1 cell
s, resulting in the upregulation of CLA expression. The switching of cytoki
ne from IL-12 to IL-4 at three cycles resulted in a marked downregulation o
f CLA. The transcript levels of 16 glycosyltransferases and P-selectin glyc
oprotein ligand-1, all considered to be potentially involved in CLA synthes
is, were determined after each cycle. The level of CLA expression was well
correlated with the amounts of alpha 1,3-fucosyltransferase VII and beta 1,
4-galactosyltransferase I. Both were upregulated by IL-12 and downregulated
by IL-4. In particular, alpha 1,3-fucosyltransferase VII levels decreased
markedly in the presence of IL-4. P-selectin glycoprotein ligand-1 and Core
2 beta 1,6-N-acetylglucosaminyltransferase were progressively up-regulated
by repeated IL-12 stimulation, but they were not downregulated by IL-4. Th
e transcript levels of some genes examined were constitutive without any co
rrelation to CLA expression. These results suggest that the level of CLA ex
pression is determined by alpha 1,3-fucosyltransferase VII and beta 1,4-gal
actosyltransferase I, the other enzymes merely participating in the synthes
is of CLA. In peripheral blood mononuclear cells, IL-12 and IL-4 profoundly
upregulated and downregulated the alpha 1,3-fucosyltransferase VII transcr
ipts, respectively, but not the beta 1,4-galactosyltransferase I ones, with
in only 2 h of in vitro culture. This suggested that alpha 1,3-fucosyltrans
ferase VII is transcriptionally regulated directly by IL-12 and IL-4.