The infusion of ozone into the inflamed quarter of cows with clinical masti
tis was performed and the efficacy of ozone therapy was evaluated. Ozone wa
s infused into the inflamed quarter via a teat canal using ozone gas genera
ting equipment. Nineteen Holstein cows with acute clinical mastitis were di
vided into two groups: 15 cows treated with ozone therapy, and 4 cows treat
ed with antibiotic therapy. Systemic and local clinical signs, California M
astitis Test scores, the mastitis causing pathogens, electronic conductivit
y of milk, and somatic cell counts in milk from ozone- and antibiotic-treat
ed quarters, were compared between the groups. Sixty percent (9/15) of cows
with acute clinical mastitis treated with ozone therapy, did not require a
ny antibiotics for recovery. This newly developed ozone therapy method was
proven to be effective, safe, and cost effective, and carries no risk of dr
ug residues in milk.