A sensitive method for the detection of foot and mouth disease virus by insitu hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification

Authors
Zhang, ZD Kitching, P
Citation
Zd. Zhang et P. Kitching, A sensitive method for the detection of foot and mouth disease virus by insitu hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification, J VIROL MET, 88(2), 2000, pp. 187-192
Citations number
13
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGICAL METHODS
ISSN journal
01660934 → ACNP
Volume
88
Issue
2
Year of publication
2000
Pages
187 - 192
Database
ISI
SICI code
0166-0934(200008)88:2<187:ASMFTD>2.0.ZU;2-S
Abstract
An in situ hybridisation technique, based on oligodeoxynucleotide probes an d tyramide signal amplification, is described for the detection of foot and mouth disease virus RNA in infected cells. Biotinylated oligodeoxynucleoti de probes, with and without tyramide signal amplification, were compared. T he tyramide signal amplification detection enhances by at least 100-fold th e sensitivity of in situ hybridisation. (C) 2000 Elsevier Science B.V. All rights reserved.