HLA-A2402-RESTRICTED AND TUMOR-SPECIFIC CYTOTOXIC T-LYMPHOCYTES FROM TUMOR-INFILTRATING LYMPHOCYTES OF A CHILD WITH WILMS-TUMOR

The existence of specific immunity against pediatric tumors is not wel l studied. We report here the CD3(+)CD4(-)CD8(+) cytotoxic T lymphocyt e (CTL) line established from tumor-infiltrating lymphocytes (TIL) of a 3-mo-old child with Wilms' tumor. This CD3(+)CD4(-)CD8(+) CTL line s howed cytotoxicity against the HLA-A2402(+) tumor cells including the autologous tumor, adenocarcinomas from various organs (colon, stomach, lung, and ovary), and an esophageal squamous cell carcinoma. No other cell lines examined, including HLA-A2402(-) tumors, K562 cells, or HL A-A2402(+) normal cells were lysed by this CTL line. This CTL line rec ognized an HLA-A2402(-) ovarian adenocarcinoma transfected with HLA-A2 402 cDNA. These results suggest the existence of HLA-A2402-restricted and tumor-specific CTL at the tumor site of a child with Wilms' tumor.