Retrovirus vector containing wild type p53 gene and its effect on human glioma cells

Authors
Hlavaty, J Tyukosova, S Bies, J Hlubinova, K Altaner, C
Citation
J. Hlavaty et al., Retrovirus vector containing wild type p53 gene and its effect on human glioma cells, NEOPLASMA, 47(4), 2000, pp. 204-211
Citations number
47
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
NEOPLASMA
ISSN journal
00282685 → ACNP
Volume
47
Issue
4
Year of publication
2000
Pages
204 - 211
Database
ISI
SICI code
0028-2685(2000)47:4<204:RVCWTP>2.0.ZU;2-5
Abstract
A retroviral vector containing wild-type p53 tumor suppressor gene (wt-p53) under the control of viral LTR sequences was constructed and transfected i nto packaging cell line GP+envAm12. Virus producing single cell clone GP+en vAm12/p53clC8 (8 x 10(5) cfu/ml, determined on NIH 3T3 cells) was isolated and used to transfer wt-p53 gene into human glioma cell lines in vitro. Dec reased viability in p53-infected cells as compared to uninfected or empty v irus infected cells was observed.