Pattern of long-distance projections from fetal hippocampal field CA3 and CA1 cell grafts in lesioned CA3 of adult hippocampus follows intrinsic character of respective donor cells
Ak. Shetty et al., Pattern of long-distance projections from fetal hippocampal field CA3 and CA1 cell grafts in lesioned CA3 of adult hippocampus follows intrinsic character of respective donor cells, NEUROSCIENC, 99(2), 2000, pp. 243-255
Fetal hippocampal grafts transplanted to the lesioned CA3 of adult hippocam
pus can extend axonal projections to many regions of the host brain. Howeve
r, the identity of grafted cells that project to specific host regions is u
nknown. We hypothesize that the pattern of long-distance axonal projections
from distinct fetal hippocampal cells grafted to lesioned CA3 is specified
by the intrinsic nature of respective donor cells rather than characterist
ics of the host graft region. We grafted fetal hippocampal CA3 or CA1 cells
into kainic acid lesioned CA3 of adult hippocampus at four days post-lesio
n. Neurons projecting to either the contralateral hippocampus or the ipsila
teral septum were then measured in these grafts at four months post-graftin
g using Fluoro-Gold and DiI tract tracing. CA3 grafts located close to the
degenerated CA3 cell layer showed a high propensity for establishing projec
tions into the contralateral hippocampus (commissural projections) compared
to similarly located CA1 grafts, which exhibited negligible commissural pr
ojections. Similar distinction was observed between the two graft types eve
n when they were located only partially in the lesioned CA3. Among CA3 graf
ts, those placed near the degenerated CA3 cell layer established significan
tly greater commissural projections than those placed only partially in the
CA3 region. Septal projections, in contrast, were robust from both CA3 and
CA1 grafts. This differential projection pattern between CA3 and CA1 graft
s resembles projections of CA3 and CA1 cells in intact hippocampus.
These results demonstrate that the intrinsic character of grafted fetal cel
ls determines the type of efferent projections from fetal grafts into diffe
rent targets in the lesioned adult host brain. However, the extent of effer
ent projections from specific grafts is also influenced by the location of
grafted cells within the host region. Thus, graft-mediated appropriate reco
nstruction of damaged circuitry in the lesioned brain may require grafting
of homotopic donor cells. Further, the robust and specific projections obse
rved from CA3 grafts is likely beneficial for functional recovery of hippoc
ampus following CA3 injury and hence of significance towards developing a g
raft-mediated therapy for human temporal lobe epilepsy. (C) 2000 IBRO. Publ
ished by Elsevier Science Ltd. All rights reserved.