Imaging Tc-99m-labeled FGF-1 targeting in rats

Recombinant human acidic fibroblast growth factor (FGF-1) was radiolabeled with Tc-99m by the HYNIC method. The Tc-99m-FGF-1 retained its representati ve molecular mass, heparin affinity, cellular binding to both law (Kd = 9.5 nM) and high (Kd = 125 pM) affinity sites, and mitogenic activity. Gamma c amera imaging after intravenous dosing in rats confirmed high liver and kid ney binding. Heparin significantly decreased Tc-99m-FGF-1 liver uptake and increased urinary excretion. These studies illustrate a new method for imag ing FGF-1 targeting under various conditions. NUCL MED BIOL 27;4:407-414, 2 000. (C) 2000 Elsevier Science Inc. All rights reserved.