A NOVEL METHOD FOR INDUCTION AND DETECTION OF ANAPHYLACTIC REACTION USING THE MOUSE ABDOMINAL-WALL (AW METHOD)

We found that an antigen-specific anaphylaxis was induced by antigen c hallenge to the abdominal wall, ear auricle, or subcutaneous tissue in mice sensitized 9 days previously with antigen and adjuvant. The anap hylactic reaction was detected by vascular permeability at the injecte d site 7 minutes after challenge, which was the best time for estimati on. A novel method (AW method) for induction and detection of the anap hylactic reaction in mice was established using the abdominal wall as the challenge site. This method could detect the anaphylactic response in mice 1 to 3 weeks after sensitization. The increase in vascular pe rmeability was completely inhibited by administration of diphenhydrami ne.