THE EFFICACY OF ANTIOXIDANTS ADMINISTERED DURING LOW-TEMPERATURE STORAGE OF WARM ISCHEMIC KIDNEY TISSUE-SLICES

Accumulation of products of lipid peroxidation (malondialdehyde, conju gated dienes, lipid peroxides, and Schiff bases) was evaluated in rabb it kidney cortex slices made ischemic for 60 min followed by 18 h stor age at 5 degrees C in UW Na gluconate solution and 210 min normothermi c reoxygenated incubation. In addition, the effect of adding Trolox (1 mM), deferoxamine (1 mM) and ascorbate (1 mM), as supplemental antiox idants to the UW gluconate solution was evaluated. Lipid peroxidation was slightly increased after hypothermic storage compared to slices su bjected to ischemia alone but was not significantly different than isc hemic slices during subsequent incubation at normothermia. The additio n of either deferoxamine or Trolox to the storage solution substantial ly reduced lipid peroxidation both during hypothermic storage and subs equent to normothermic incubation Ascorbate had a mild prooxidant effe ct as a sole additive to the UW gluconate solution but was clearly pro oxidant when combined with either deferoxamine or Trolox. These result s suggest that supplemental antioxidants added to the UW gluconate sol ution under conditions analogous to machine perfusion preservation hav e a potential role in reducing oxidative stress in kidney tissues harv ested after warm ischemia and that hypothermia may be a valuable adjun ct to resuscitative therapeutic regimens developed for salvage of isch emic kidneys for transplantation. (C) 1997 Academic Press.