REINTERPRETATION OF G-BANDED COMPLEX KARYOTYPES BY FLUORESCENCE IN-SITU HYBRIDIZATION WITH CHROMOSOME-SPECIFIC DNA PAINTING PROBES AND ALPHA-SATELLITE CENTROMERE-SPECIFIC DNA PROBES IN MALIGNANT HEMATOLOGICAL DISORDERS

In this study, we have performed fluorescence in situ hybridization (F ISH) with chromosome-specific DNA painting probes 1, 2, 3, 4, 6, 8, an d 12 and centromere-specific DNA probes 7, 10, 12, 17, 18, and X after G-banding on the same metaphase spreads from four patients with malig nant hematological disorders to more precisely interpret their complex karyotypes, The findings demonstrated that the application of combine d G-banding and FISH can more accurately explain complex karyotypes of hematological malignancies. FISH can detect not only the origin of ma rker chromosomes, but also the complex rearrangements that cannot be i dentified by routine banding techniques. This approach is very importa nt to complement the cytogenetic analysis of malignant disorders and t o evaluate the role of chromosome change in the development, progressi on, and prognosis of tumors. (C) 1997 Wiley-Liss, Inc.