Effects of vitamin E and prostaglandin E-2 on expression of CREB1 and CREB2 proteins by human T lymphocytes

Both prostaglandins (PGs) and vitamin E are known to deeply affect immune r esponses. It is shown here that they both influence T cell-mediated immunit y through reciprocal interference on the expression of cyclic-AMP responsiv e element binding (CREB) family proteins. CREB1 protein of human T lymphocy tes was significantly modulated by a brief treatment of 5 to 10 min with PG E(2). On the contrary, vitamin E appeared to be ineffective on the CREB1 be havior, while it abolished the PGE(2)-induced modulation of this protein. T he CREB2 protein expression was also affected by PGE(2) treatment, but a lo nger period of incubation (>20 min) was needed to observe these changes. Vi tamin E showed a strong enhancing effect on CREB2 that was partially revers ed by the subsequent treatment with PGE(2). Our results support the idea th at there is reciprocal interference between PGE(2) and vitamin E on PGE(2)- induced signals in T lymphocytes. These data are in agreement with the repo rts concerning different cell systems and experimental conditions.