Rapid purification of His(6)-tagged Bacillus subtilis core RNA polymerase

Authors
Anthony, LC Artsimovitch, I Svetlov, V Landick, R Burgess, RR
Citation
Lc. Anthony et al., Rapid purification of His(6)-tagged Bacillus subtilis core RNA polymerase, PROT EX PUR, 19(3), 2000, pp. 350-354
Citations number
15
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PROTEIN EXPRESSION AND PURIFICATION
ISSN journal
10465928 → ACNP
Volume
19
Issue
3
Year of publication
2000
Pages
350 - 354
Database
ISI
SICI code
1046-5928(200008)19:3<350:RPOHBS>2.0.ZU;2-6
Abstract
Bacillus subtilis core RNA polymerase, containing a His(6)-fusion to the C- terminus of the beta' subunit, was isolated by Ni-NTA, Superdex 200 gel fil tration, and Mono Q anion-exchange chromatography. The purified core enzyme was shown to be free of the major sigma factor sigma(A) and the transcript ion factors NusA and GreA. The purification procedure can be completed with in 1 working day, is scalable, and yields highly purified and active core R NA polymerase. (C) 2000 Academic Press.