Steady-state and maximum chlorophyll fluorescence responses to water stress in grapevine leaves: A new remote sensing system

A new fluorimeter built at Orsay allowed us to measure at a distance of up to 6 m both the stead-state and the maximum chlorophyll fluorescence. This instrument has been applied continuously during 17 days of water stress dev elopment to follow the chlorophyll fluorescence parameters of a potted grap evine. Gas-exchange rates for H2O and CO2 and chlorophyll fluorescence para meters of the same leaf were recorded concurrently. It was shown that: (1) Under well-watered conditions, before noon, a correlation was found between net photosynthetic rate and the rate of electron transport calculated from fluorescence measurements. After several hours of high light exposure, CO2 assimilation (A) started to decrease more than the rate of electron transp ort (ETR). Under drought conditions, the above-mentioned correspondence was lost: when A almost vanished due to high stomatal closure, the ETR was sti ll about 50% of the control value. It is suggested that under these conditi ons, the ratio of photorespiration to CO2 assimilation increased (2) Light response of the quantum yield of ETR became increasingly different between, morning and afternoon as water stress progressed, thus serving as a good in dicator of plant water status. (3) A simple fluorescence parameter, Fs, acc urately reflected the plant physiological state. Over the range of light in tensities used in this study, this parameter changed in parallel with irrad iance in well-watered plants. With increasing water stress, Fs changed in o pposite direction to irradiance changes. The response of Fs to rapid change s in irradiance was fast (within seconds). The potential of this parameter for remote sensing of water stress is discussed. (C) Elsevier Science Inc., 2000.