Development of biosensors based on hexacyanoferrates

Ferric and copper hexacyanoferrates (PB and CuHCF, respectively) were elect rodeposited on glassy carbon electrodes providing a suitable catalytic surf ace for the amperometric detection of hydrogen peroxide. Additionally gluco se oxidase was immobilized on top of these electrodes to form glucose biose nsors. The biosensors were made by casting glucose oxidase-Nafion layers on to the surface of the modified electrodes. The operational stability of the films and the biosensors were evaluated by injecting a standard solution ( 5 mu M H2O2 for PB, 5 mM H2O2 for CuHCF and 1.5 mM glucose for both) over 5 -10 h in a now-injection system with the electrodes polarized at - 50 (PB) and -200 mV (CuHCF) versus Ag/AgCl, respectively. The glucose biosensors de monstrated suitability for glucose determination: 0.0-2.5 mM (R-2 = 0.9977) for PB and 0.0-10 mM (R-2 = 0.9927) for CuHCF, respectively. The visualiza tion of the redox catalyst modifiers (PB and CuHCF films) was presented by scanning electron micrographs. (C) 2000 Elsevier Science B.V. All rights re served.