Determination of ciprofloxacin in human urine and serum samples by solid-phase spectrofluorimetry

A method for the determination of trace amounts of ciprofloxacin has been d eveloped, based on solid-phase spectrofluorimetry. The relative fluorescenc e intensity of ciprofloxacin fixed on Sephadex SP C-25 gel was measured dir ectly after packing the gel beads in a 1-mm silica cell, using a solid-phas e attachment. The wavelengths of excitation and emission were 272 and 448 n m, respectively. Using a sample volume of 1000 mi, the linear concentration range of application was 0.3 - 10.0 ng.ml(-1) of ciprofloxacin, with a R.S .D. of 1.2% (for a level of 4.0 ng.ml(-1)) and a detection limit of 0.1 ng. ml(-1). The method was applied to the determination of ciprofloxacin in hum an urine and serum samples. It was validated applying the standard addition methodology and using HPLC as a reference method. Recovery levels of the m ethod reached 100% in all cases. (C) 2000 Elsevier Science B.V. All rights reserved.