BACKGROUND: The effect of storing packed RBCs suspended in 300 mL of an alk
aline, experimental additive solution (EAS 64) was explored.
STUDY DESIGN AND METHODS: RBC units prepared from blood collected from heal
thy donors into CPD were WBC reduced and stored for 10 weeks under blood ba
nk conditions after the addition of 300 mt of EAS 64 (adenine, 2 mM; dextro
se, 50 mM; mannitol, 20 mM; NaCl, 75 mM; Na2HPO4, 9 mM). For comparison, no
n-WBC-reduced units from the same donors were stored in a different additiv
e solution (AS-1, Baxter Healthcare) for 6 weeks. Standard methods were use
d for the in vitro assays. The 24-hour in vivo recoveries were measured by
using Cr-51- and Tc-99m-labeled RBCs.
RESULTS: Mean recovery in the EAS 64 units after 10 weeks was 84 +/- 8 perc
ent, the same as in the AS-1 units stored for 6 weeks. For EAS 64 and AS-1
units, respectively, the ATP of the RBCs was 85 percent and 64 percent of t
he initial value, hemolysis was 0.43 percent and 0.63 percent, supernatant
potassium was 24 mEq per L and 44 mEq per L, and the morphologic index was
98 and 71.
CONCLUSION: RBCs suspended in 300 mt of EAS 64 can be stored satisfactorily
for 10 weeks. Longer RBC storage should reduce outdating, increase availab
ility of transfusions in remote locations, and improve the efficiency of au
tologous donor programs.