Isolation and characterization of porcine circovirus type 2 from pigs showing signs of post-weaning multisystemic wasting syndrome in the Netherlands

Citation
Gj. Wellenberg et al., Isolation and characterization of porcine circovirus type 2 from pigs showing signs of post-weaning multisystemic wasting syndrome in the Netherlands, VET Q, 22(3), 2000, pp. 167-172
Citations number
19
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
VETERINARY QUARTERLY
ISSN journal
01652176 → ACNP
Volume
22
Issue
3
Year of publication
2000
Pages
167 - 172
Database
ISI
SICI code
0165-2176(200007)22:3<167:IACOPC>2.0.ZU;2-D
Abstract
Pigs with wasting syndrome were examined for macroscopic and histopathologi cal lesions, and for porcine circovirus (PCV), Histopathological lesions we re comparable to those previously documented for post-weaning multisystemic wasting syndrome (PMWS), In addition, in seven out often examined PMWS-aff ected pigs focal-to-slight mononuclear meningitis and focal cerebral mononu clear infiltrates (4 out of 10) were observed. A virus was isolated from or gans and sera from pigs showing wasting syndrome. An immunoperoxidase monol ayer assay and an indirect immunofluorescence assay were performed on the i nfected PK-15 and Dulac cell cultures, respectively, and both assays indica ted the presence of PCV type 2 (PCV2), The nested-polymerase chain reaction (nPCR) technique, based on the use of PCV2 specific oligonucleotides, reve aled specific amplified products of 481 bp, Nucleotide sequence analysis of the entire genome of the Dutch PCV isolate 24657 NL showed a homology with known nucleotide sequences of porcine PCV type 1 (PCV1) and PCV2 isolates of 77.1% and >96%, respectively. This is the first report of the isolation and characterization of PCV2 in PMWS-affected pigs in the Netherlands.