Gj. Wellenberg et al., Isolation and characterization of porcine circovirus type 2 from pigs showing signs of post-weaning multisystemic wasting syndrome in the Netherlands, VET Q, 22(3), 2000, pp. 167-172
Pigs with wasting syndrome were examined for macroscopic and histopathologi
cal lesions, and for porcine circovirus (PCV), Histopathological lesions we
re comparable to those previously documented for post-weaning multisystemic
wasting syndrome (PMWS), In addition, in seven out often examined PMWS-aff
ected pigs focal-to-slight mononuclear meningitis and focal cerebral mononu
clear infiltrates (4 out of 10) were observed. A virus was isolated from or
gans and sera from pigs showing wasting syndrome. An immunoperoxidase monol
ayer assay and an indirect immunofluorescence assay were performed on the i
nfected PK-15 and Dulac cell cultures, respectively, and both assays indica
ted the presence of PCV type 2 (PCV2), The nested-polymerase chain reaction
(nPCR) technique, based on the use of PCV2 specific oligonucleotides, reve
aled specific amplified products of 481 bp, Nucleotide sequence analysis of
the entire genome of the Dutch PCV isolate 24657 NL showed a homology with
known nucleotide sequences of porcine PCV type 1 (PCV1) and PCV2 isolates
of 77.1% and >96%, respectively. This is the first report of the isolation
and characterization of PCV2 in PMWS-affected pigs in the Netherlands.