Formation of aliphatic and aromatic alpha-hydroxy ketones by Zygosaccharomyces bisporus

Citation
F. Neuser et al., Formation of aliphatic and aromatic alpha-hydroxy ketones by Zygosaccharomyces bisporus, Z NATURFO C, 55(7-8), 2000, pp. 560-568
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES
ISSN journal
09395075 → ACNP
Volume
55
Issue
7-8
Year of publication
2000
Pages
560 - 568
Database
ISI
SICI code
0939-5075(200007/08)55:7-8<560:FOAAAA>2.0.ZU;2-E
Abstract
The wild-type yeast strain Zygosaccharomyces bisporus CBS 702 produced alph a-hydroxyketones (acyloins) from amino acid precursors after transamination to the corresponding 2-oxo acids. The key enzyme of the subsequent decarbo xylation and C-C bond forming reaction, pyruvate decarboxylase (PDC), was e xamined for its substrate- and stereo-specificity. A wide variety of satura ted and unsaturated aliphatic and aromatic aldehydes was successfully conve rted to acyloins. 19 of the biotransformation products identified had not b een reported as natural substances before. Product yields were strongly aff ected by substrate structure.