Levels of house-dust-mite allergen in homes of nonallergic people in Pavia, Italy

Background: HDM distribution varies between geographic areas and may be aff ected by housing characteristics. We quantified Der p 1 and Der f 1 and ass essed the relationships between their levels and housing characteristics in homes of nonallergic subjects. Methods: Der f 1 and Der p 1 were measured by ELISA in dust samples from li ving-room floors and mattresses of 44 homes of nonallergic subjects in Pavi a. Information about household characteristics was obtained by questionnair e. Results: Der p 1 and Der f 1 concentrations (mu g/g dust, median) were 0.34 and 7.8 on mattresses, and 0.15 and 0.83 on living-room floors. Higher Der f 1 levels on mattresses were associated with synthetic pillows (P < 0.05) , and (only when expressed as mu g/m(2)) with bedding washing temperature o f less than or equal to 60 degrees C (P < 0.05). Der f 1 levels were higher on the living-room floors of homes located on lower (less than or equal to first) floors (P < 0.05). Good correlations were found between Der p 1 and Der f 1 expressed as mu g/g dust and mu g/m(2) on both mattresses and livi ng-room floors. Conclusions: In homes of nonallergic subjects in northern Italy, Der f 1 ex ceeded the threshold for sensitization in a high proportion of mattress sam ples.