Peroxidase enhanced lanthanide luminescence - a new technique for the evaluation of bioassays

A new technique for the enzyme-amplified lanthanide luminescence (EALL) qua ntification of peroxidase in bioassays is introduced. Several phenolic pero xidase substrates may be used. These are dimerised in the course of the enz ymatic reaction and subsequently form luminescent ternary complexes with te rbium(III) EDTA. The luminescence signals are enhanced by addition of CsCl and can be read out in a time resolved type of measurement. With the substr ate p-hydroxyphenylpropionic acid, a detection limit for horseradish peroxi dase of 2 x 10(-12) mol dm(-3) could be achieved.