Hyperproduction of polyesters consisting of medium-chain-length hydroxyalkanoate monomers by strain Pseudomonas stutzeri 1317

Pseudomonas stutzeri strain 1317 was found to grow on various fatty acids, alcohols, diols, as well as glucose and gluconate for the synthesis of poly hydroxyalkanoates (PHA) with various monomer units. The PHA monomer structu res were dependent on the type of fatty acids and alcohols, as well as the diols in the culture media. Only even number monomers, such as 3-hydroxyhex anoate (HHx), 3-hydroxyoctanoate (HO) and 3-hydroxydecanoate (HD), were acc umulated when even numbered fatty acids, alcohols, glucose and gluconate, a s well as diol were used as carbon sources. Odd numbered fatty acids and od d numbered alcohols led to the formation of odd numbered monomers, such as 3-hydroxyvalerate (HV), 3-hydroxyheptanoate (HHp), 3-hydroxynonanoate (HN) and 3-hydroxyundecanoate (HU). The strain tolerated up to 1.5% of ethanol a nd made 8.3% of PHA when growth was conducted in 1.2% of ethanol. PHA forme d up to 77% of cell dry weight when the strain was grown in tridecanoate. P HA synthesis was highly dependent on the nitrogen source. A depletion in ni trogen supply immediately resulted in PHA accumulation in cells grown in th e glucose mineral medium.