E. Haneda et al., Biochemical characterization of casein kinase II as a protein kinase responsible for stimulation of HIV-1 protease in vitro, BIOC BIOP R, 275(2), 2000, pp. 434-439
Citations number
18
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
The physiological significance of casein kinase II (CK-II) on the protease
(PR) activity of recombinant HIV-1 PR (rPR) was biochemically investigated
in vitro. We found that (i) the purified rPR (pll) functions as a phosphate
acceptor of CK-II; (ii) the PR activity of rPR is stimulated approximately
2.9-fold after its full phosphorylation by recombinant human CK-II (rhCK-I
I) in a manner similar to that observed for recombinant HIV-1 reverse trans
criptase (rRT); and (iii) this stimulation is completely inhibited by two p
olyphenol-containing anti-oxidant compounds [quercetin and epigallo-catechi
n gallate (EGCG)] at 0.1 mu M or a glycyrrhetinic acid derivative (oGA) and
catechin at 10 mu M without significant effect on the PR activity of rPR.
These results suggest that (i) CK-II may be a host mediator responsible for
stimulation of PR and RT in HIV-1-infected cells; and (ii) the selective i
nhibition of the CK-II-mediated stimulation of HTV-1 PR and RT by potent CK
-II inhibitors may be involved in their anti-HIV-1 effects at the cellular
level. (C) 2000 Academic Press.