Effects of modification of the hydrophobic C-1-C-16 segment of tautomycin on its affinity to type-1 and type-2A protein phosphatases

Among the naturally occurring toxins that are known to have specific inhibi tory effects on type-1 and type-2A protein phosphatases (PP1 and PP2A), tau tomycin (TM) is unique in that it exhibits significantly higher affinity to PP1 than to PP2A. The ratio of the dissociation constant for the PP1-TM in teraction to that for the PP2A-TM interaction (the PP1/PP2A ratio) is 0.01- 0.03. The aim of the present study was to evaluate the possible contributio ns of the C-1-C-16 segment of TM to the affinity characteristics of the tox in. The relatively hydrophobic segment contains a spiroketal motif whose en antiomeric form is present in okadaic acid (OA), which exhibits exceedingly higher affinity to PP2A than to PP1. We therefore synthesized two TM analo gues: TM1 in which the side chains of the spiroketal motif of TM were remov ed but its absolute configuration was retained, and TM2 in which the spirok etal motif of TM1 was replaced with its enantiomeric form. The effects of T M, TM1 and TM2 on the activities of the native catalytic subunits of PP1 (P P1C) and PP2A and a recombinant gamma isoform of PP1 (PP1 gamma) were exami ned. The PP1/PP2A ratio determined thereby was 0.2-0.5 for TM1 and 5-10 for TM2. Both the presence of the side chains and the stereochemistry of the s piroketal moieties may be major determining factors for the affinity charac teristics of TM. We also show that a monoclonal antibody raised against OA binds to TM2 albeit with much lower affinity than to OA, whereas it exhibit s no measurable affinities to TM and TM1.