Ca2+ is a ubiquitous second messenger in the signal transduction pathway(s)
by which stomatal guard cells respond to external stimuli. Increases in gu
ard-cell cytosolic free Ca2+ concentration ([Ca2+](eyt)) hare been observed
in response to stimuli that cause both stomatal opening and closure. In ad
dition, several important components of Ca2+-based signalling pathways have
been identified in guard cells, including the cADP-ribose and phospholipas
e C/Ins(1,4,5)P-3-mediated Ca2+-mobilizing pathways. The central role of st
imulus-induced increases in [Ca2+](eyt) in guard-cell signal transduction h
as been clearly demonstrated in experiments examining the effects of modula
ting increases in [Ca2+](eyt) on alterations in guard-cell turgor or the ac
tivity of ion channels that act as effecters in the guard-cell turgor respo
nse. In addition, the paradox that Ca2+ is involved in the transduction of
signals that result in opposite end responses (stomatal opening and closure
) might be accounted for by the generation of stimulus-specific Ca2+ signat
ures, such that increases in [Ca2+](eyt) exhibit unique spatial and tempora
l characteristics.