A novel approach to provide, thermally sensitive neutral solutions based on
chitosan/polyol salt combinations is described. These formulations possess
a physiological pH and can be held liquid below room temperature for encap
sulating living cells and therapeutic proteins; they form monolithic gels a
t body temperature. When injected in vivo the liquid formulations turn into
gel implants in situ, This system was used successfully to deliver biologi
cally active growth factors in vivo as well as an encapsulating matrix for
living chondrocytes for tissue engineering applications. This study reports
for the first time the use of polymer/polyol salt aqueous solutions as gel
ling systems, suggesting the discovery of a prototype for a new family of t
hermosetting gels highly compatible with biological compounds. (C) 2000 Els
evier Science Ltd. All rights reserved.