Mutational analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitrosomonas sp strain ENI-11

Authors
Yamagata, A Hirota, R Kato, J Kuroda, A Ikeda, T Takiguchi, N Ohtake, H
Citation
A. Yamagata et al., Mutational analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitrosomonas sp strain ENI-11, BIOS BIOT B, 64(8), 2000, pp. 1754-1757
Citations number
18
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
64
Issue
8
Year of publication
2000
Pages
1754 - 1757
Database
ISI
SICI code
0916-8451(200008)64:8<1754:MAOTMH>2.0.ZU;2-T
Abstract
The ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11 contains thr ee copies of the hao gene (hao(1), hao(2), and hao(3)) coding for hydroxyla mine oxidoreductase (HAO). Three single mutants (hao(1)::kan, hao(2)::kan, or hao(3)::kan) had 68 to 75% of the wild-type growth rate and 58 to 89% of the wild-type HAO activity when grown under the same conditions. A double mutant (hao(1)::kan and hao(3)::amp) also had 68% of the wild-type growth a nd 37% of the wild-type HAO activity.