A. Oosterveld et al., Characterization of arabinose and ferulic acid rich pectic polysaccharidesand hemicelluloses from sugar beet pulp, CARBOHY RES, 328(2), 2000, pp. 185-197
Pectic polysaccharides were extracted from sugar beet pulp to yield fractio
ns representing homogalacturonans, rhamnogalacturonans, arabinans and relat
ively small amounts of glucomannans and xyloglucans. The homogalacturonans
had an apparent molecular weight of 21 kDa and contained relatively high am
ounts of methyl esters and relatively low amounts of acetyl groups as compa
red with the ramified 'hairy' regions. Three populations which originated f
rom the ramified 'hairy' regions of pectin were distinguished. Two of these
were rhamnogalacturonans with high apparent molecular weights of 1300 and
120 kDa, respectively. These populations had a high Ara and ferulic acid co
ntent. Despite the high neutral sugar content, these rhamnogalacturonans st
rongly bound to a DEAL column. The third population which originated from t
he ramified 'hairy' regions was a neutral population, which did not interac
t with the DEAE column and had a low apparent molecular weight and a high A
ra and ferulic acid content. The arabinan side-chains of the rhamnogalactur
onans were heavily branched in all populations. Enzymatic degradation of th
e xyloglucans showed similarities with apple xyloglucans with respect to th
e substitution with Fuc and Gal. (C) 2000 Elsevier Science Ltd. All rights
reserved.