Structure of an O-acetylated acidic O-specific polysaccharide of Proteus vulgaris O46

Authors
Perepelov, AV Senchenkova, SN Torzewska, T Bartodziejska, B Shashkov, AS Rozalski, A Knirel, YA
Citation
Av. Perepelov et al., Structure of an O-acetylated acidic O-specific polysaccharide of Proteus vulgaris O46, CARBOHY RES, 328(2), 2000, pp. 229-234
Citations number
16
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
328
Issue
2
Year of publication
2000
Pages
229 - 234
Database
ISI
SICI code
0008-6215(20000908)328:2<229:SOAOAO>2.0.ZU;2-4
Abstract
An acidic O-specific polysaccharide was obtained by mild acid degradation o f the lipopolysaccharide of Proteus vulgaris O46 and studied by chemical me thods (O-deacetylation, sugar and methylation analyses, partial solvolysis) and H-1 and C-13 NMR spectroscopy. Solvolysis of the O-deacetylated polysa ccharide with trifluoromethanesulfonic acid resulted in a alpha-D-GlcpNAc-( 1 --> 3)-D-GlcA disaccharide that demonstrated the usefulness of this reage nt for selective cleavage of heteropolysaccharides. The following structure for the polysaccharide was established: --> 4)-alpha-D-Glcp6Ac(1 --> 3)-beta-D-GlcpA4Ac-(1 --> 3)-alpha-D-GlcpNAc-( 1 --> 3)-beta-D-GlcpA4Ac-(1 --> where the degree of O-acetylation is similar to 65% at position 6 of Glc an d 80-95% at position 4 of GlcA residues. (C) 2000 Elsevier Science Ltd. All rights reserved.