Amino acid selective 'unlabelling' for residue-specific NMR assignments inproteins

Citation
Hs. Atreya et Kvr. Chary, Amino acid selective 'unlabelling' for residue-specific NMR assignments inproteins, CURRENT SCI, 79(4), 2000, pp. 504-507
Citations number
17
Categorie Soggetti
Multidisciplinary,Multidisciplinary
Journal title
CURRENT SCIENCE
ISSN journal
00113891 → ACNP
Volume
79
Issue
4
Year of publication
2000
Pages
504 - 507
Database
ISI
SICI code
0011-3891(20000825)79:4<504:AAS'FR>2.0.ZU;2-P
Abstract
A novel methodology for sequence-specific resonance assignments in proteins , using amino acid selective 'unlabelling' is presented. The strategy is ba sed on selective unlabelling of amino acid residues in uniformly or fractio nally C-13 or/and N-15 labeled proteins, which simplify the multi-dimension al heteronuclear NMR spectra. This aids in sequence-specific resonance assi gnments of both backbone and side-chain nuclei, The methodology has been de monstrated by unlabelling Lys residues in a 15 kDa calcium-binding protein from Entamoeba histolytica (Eh-CaBP).