Jm. De Gandarias et al., Subcellular distribution of pyroglutamyl-peptidase I activity in the developing rat cerebellum, DEV NEUROSC, 22(4), 2000, pp. 264-273
In this work we analyzed the activity of pyroglutamyl (pGlu)-peptidase I in
several subcellular fractions of the rat cerebellum during its development
. The aim of this study is to determine if there is a developmental redistr
ibution of this enzyme activity and if this fact could indicate changes in
the role of the enzyme as age progresses. Results show that pGlu-peptidase
I is widely distributed, but not homogeneously, in all the subcellular frac
tions that were studied, in both soluble and particulate forms. Significant
ly the distribution of the enzyme changes with development. Thus, in the so
luble synaptosomal fraction, the pGlu-peptidase I activity is low until PD9
and the activity increases significantly from PD9 to PD15, when it reaches
adult levels. In contrast, in the cytosolic fraction, the pGlu-peptidase I
activity is high from fetal day 22 to postnatal day 6, and then decreases
significantly until postnatal day 90. Copyright (C) 2000 S, Karger AG, Base
l.